[Method Information]
LC Method

General Information:
Author: Umymfor
LC Parameters (acquisition starting conditions)

Acquisition Parameters:

Runtime:        4.02 min
Slice Width:    2.5  points/s

[Pump]
Additional Pump and Oven Parameters:

pump settings
 Nr. RunTime  Flow Rate     Min/Max           Solvents
      (min)   (ml/min)      Press(bar)
   1    4.00   0.20000         0/500
                                            A: water
                                            B: acetonitrile

LC Column:     



[Pump]
LC Timetable

  Time  Function                 Value         A(%)  B(%)  C(%)  D(%)
  0.00  Flow Rate                0.20000                  
  0.00  Solvent Mix                            100.0 0.0   0.0   0.0  


[Detector]
Detector Settings
Detector1 : Agillent ICF System
Offset: 0.00, InvertSignal: 0, Slicewidth: 0.00(points/sec)
Autozero: no, Spec Start: 190.00, Spec End: 900.00, Start Delay: 0
Data Channel: 1, DoReference: no, ReferenceStart: 254.00, ReferenceWidth: 10.00
DoSaveSpectra: no, SaveInterval: 1, SaveType: 1


Data Acquisition - Signals

SIGNAL          Source          Parameters                          Autodetection Parameters
   No.                                             
    1   Agillent ICF System Wavelength: 254           
                                                           Slope(mAU/s):  20.00 Peak Par.: 5
                                                                   Threshold: 0 Smoothing: 2 
    2   micrOTOF series     Intervals: BPC,All        
                                                            Smoothing Width: 20 Min Points: 20
                                                                   Threshold: 0 Smoothing: 2 


Methods

LC Method Part: D:\Methods\lc_qtof_sample_cali.m

Autosampler Method Part: Pretreatment

MS Acquisition Part: D:\Methods\lc_tune_high_pos.m

MS Processing Part: D:\Methods\ggc.m




Hardware Setup

Hardware Setup used: "LCMS"

General Information:
Author: BDAL@DE

[System Information]
System included components:

Pumps
Pump 1 : Agillent ICF System
conneced via System

AutoSamplers
AutoSampler 1 : Agillent ICF System
conneced via System

Detectors
Detector 1 : Agillent ICF System
conneced via System

Systems
System 1 : Agillent ICF System
connected via LAN

MSs
MS 1 : micrOTOF series


System times with 1 ml/min flow (in sec):

Do Acquisition times:
From Zero Point = LC 1 Detector to:
Bruker Peak Sampling Unit    :  12.00
NMR Spectrometer             :  15.00
Fraction Collector (via NMR) :  100.00
Fraction Collector (direct)  :  40.00

Time offset to System Zero Point for:
LC 2  Detector    :  0.00
LC 3  Detector    :  10.00
Mass Spectrometer :  0.00
SPE Cartridge :  0.00

Elution volume  between SPE Cartridge and NMR :  290.00

Flow Injection times:
Times from Bruker Peak Sampling Unit to:
NMR Spectrometer             :  23.00
Mass Spectrometer            :  23.00
Fraction Collector (via NMR) :  110.00
Divert Valve(via NMR) :  3.00

Times from Autosampler to:
NMR Spectrometer  :  30.00
Mass Spectrometer :  30.00